Body fluids like pleural, ascetic/ peritoneal, synovial and cerebrospinal fluids are usually sterile, but they can get infected by different microbes, thus leading to life threatening infections. This study was aimed to identify the prevalence of common bacterial isolates in effusions; determine the antimicrobial susceptibility pattern; correlate the microbiological and cytological imprints of effusions. This is a cross sectional and prospective study conducted between January and December 2018 in a tertiary care teaching hospital among the samples received for pathological and microbiological investigations. Of the 267 different body fluids samples processed, 127 (47.6%) were found to have inflammatory cells, 98 (36.7%) with non infectious cells and 42 (15.7%) with malignant cells in cytology. Of the 127 inflammatory samples, 42 samples supported with bacterial growth. The most common pathogens isolated were Klebsiella pneumoniae (n=12;28.6%) followed by non fermenting gram negative bacilli, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and coagulase negative staphylococci. The gram negative isolates were relatively resistant to cephalosporins and quinolones; whereas gram positive isolates resistant to quinolones and aminoglycosides. Knowledge of the cytological investigations, bacteriological and antimicrobial resistant profile of sterile body fluids is necessary for clinicians for appropriate patient management.