During the last two decades, significant changes have taken place in the epidemiology of meningitis, especially due to the global availability and expanding use of Hib vaccines. We conducted an observational study with the aim was to isolate and identity of the pathogen causing meningitis in children under 5 years during January 2017 to December 2017 in Paediatrics department of Dhaka Shishu (Children) Hospital Dhaka, Bangladesh. Our study participants were clinically suspected cases of meningitis in children below 5 years of age admitted in the selected hospital. All the data were collected and recorded systematically in a questionnaire and entered into computer software SPSS (Statistical package for social sciences) version 20.0 edited and analyzed with plotted in graphs and tables. Sensitivity and specificity of Gram stain, CSF latex were also calculated. Main outcome measure (s): Results of CSF cell count, Gram stain, Culture, LAT and Blood culture were the main outcome variables. A total of 100 cases of suspected meningitis in the age group below 5 years comprised the study population. Majority of the patients belonged to ≤12 months. Male to female ratio was 1.6:1. The most common clinical presentation was fever (100.0%) with median duration of 2 days. Ninety six (96) patients had convulsion and the median duration of continuation of convulsion was 1 day. Thirty Eight patients had vomiting and median duration of continuation of vomiting was 1 day. A total of 100 clinically suspected cases of acute bacterial meningitis, laboratory confirmed cases were 36(36.0%), out of which 14(38.9%) cases were CSF culture positive, 10(27.8%) were Gram stain positive and 36(36.0%) were Latex agglutination test positive. Most frequently isolated organism were Streptoccous pneumonae 34(94.4%). Haemophilus influenzae was isolated in 2 cases. The validity of test of serology (LAT) sensitivity was 100.0% and specificity was 74.4%, Gram stain sensitivity was 71.4% and specificity 100.0% to detect capsular polysaccharide antigen in CSF. In the conclusion, we can say Meningitis is more common during infancy and is more common in males. Fever, convulsion, vomiting and lethargy were the commonest symptoms. CSF culture is the ‘Gold standard’ and positive in 38.9% cases, but Latex agglutination test was 100.0% sensitive for detection of Antigen in CSF.