Introduction: The urethral catheter is one of the most vulnerable of medical devices, having been used for urine retention on an intermittent or indwelling basis for centuries. Insertion of a catheter may carry urethral organisms into the bladder. Even with meticulous attention to maintenance of the closed system, the space between the external catheter and the urethral mucosa offers opportunity for bacterial entry directly into the bladder and are eliminated efficiently; most bacterial strains that enter the catheterized urinary tract are able to multiply to high concentrations within a day. The duration of catheterization is the most important risk factor for the development of catheter-associated bacteriuria. the present study was done to assess the risk factors, occurrence and aetiology of catheter associated urinary tract infections (CAUTI) in ICU of Dr D.Y Medical College, Hospital and Research centre, by various microbiological techniques and to determine the susceptibility pattern of isolates to commonly used antimicrobial agents for prophylaxis and empiric therapy of CAUTI in patients on indwelling catheter. Results: majority of the patients (17.3%) were in the age group of 61-70years followed by 41-50 years (16.2%), 51-60 years (16%), 21-30 (14.9%), 31-40 years (14.5%), 71-80 years (11.1%), 11-20 years (7.1%) and 81-90years (2.9%). The mean age of the patients was 49.21 ± 19.04 years. 62.5% patients were male and 37.5% were female. There was male preponderance and the M:F ratio was 1.67:1. It was observed that significantly higher number of patients (6.1%) patients had the indwelling catheter for 8-14 days. There was significant association of days of catheterization and CAUTI as per Chi-square test (p<0.05). The logistic regression analysis showed that days of catheterization were independently associated with CAUTI. Conclusion: The urinary tract of catheterized patients is highly susceptible to severe infection. This infection is associated varied microbiological aetiology. Antibiotic sensitivity pattern of the pathogen involved is also low. This along with existing underlying condition of comorbidities increases hospitalization, medication, morbidity and also adds to the financial burden. Therefore, it is imperative to carry out microbiological testing to determine aetiology and ascertain effective antibiotics. Emphasis should also be made on reducing the duration of catheterization in order to reduce the incidence of catheter-related UTI. Hospital-wide surveillance program and appropriate catheter care protocols should be developed and implemented from evidence-based protocol.