Introduction: Candida is a genus of yeast which is the most common cause of fungal infection. Candida albicans is most important fungal opportunistic pathogen. The candiduria refers to the presence of yeast in urine either by the microscope observation of budding pseudohyphae, or by the growth of fungi in culture. Aim: To find the prevalence of candiduria in tertiary care Centre. Materials and Method: This was a study conducted at Saveetha medical college from January 2018 to December 2018, the total number of urine sample collected were 10194 out of which 2500 showed various microbial growth, out of which 50 Candida strains were isolated. Speciation was done according to standard protocol. Result: The prevalence of candiduria caused by Candida non-albicans species (1.24%) is more than that caused by Candida albicans species (0.76%). The prevalence is more in female (66%) than male (34%). Among the age group the prevalence is more in the age group between 21-30 years (50%). Candiduria cases are common among the NON-ICU cases with 92%. Candiduria is prevalent among the non-diabetic patients with 92%. Conclusion: Urinary tract candidiasis is known as the most frequent nosocomial fungal infection worldwide. Candida albicans is the most common cause of nosocomial fungal urinary tract infections; however, a rapid change in the distribution of Candida species is undergoing. Simultaneously, the increase of urinary tract candidiasis has led to the appearance of antifungal resistant Candida species.