This cross-sectional descriptive study was conducted at a health care facility in a metropolitan city to determine the socio-demographic profile of HIV sero-positive individuals. Written informed consent was obtained from 338 HIV sero-positive persons (173 females; 51.19%; 165 males; 48.81%), aged 18-60 years. Each participant was interviewed and socio-demographic profile was recorded on a pre-validated formatted questionnaire. The mean age of female participants was 35.94  8.07 years (95% CI: 34.63–37.15 years), while that for males was 39.27  8.67 years (95% CI: 37.95–40.59 years), exhibiting highly significant (p=0.0003) gender difference. Most participants belonged to middle and lower middle socio-economic classes. The gender differences were significant among the college educated (p=0.049), the unemployed (p=0.0009), semi-skilled workers (p<0.0001) and skilled workers (p<0.0001). Likewise, significant gender differences were found among participants who were single (p=0.002), married (p=0.007) and those who were widowed/separated/divorced (p<0.0001). Focused intervention for the HIV negative spouses will reduce the possibility of female sero-positivity and mother-to-child transmission of HIV. The findings of this study reveal that HIV-related interventions need to be focused on socio-demographic sub-groups, such as, housewives, semi-skilled and skilled workers and males with higher literacy levels