Gastrointestinal lesions are the major group of specimens received in the surgical pathology department. This includes endoscopic biopsies from gastric and duodenal mucosa, appendicectomies, cholecystectomies and colonoscopy biopsies. This study was a retrospective analysis done between January 2015 and December 2017 which included 838 biopsies, both mucosal and resected specimens. These biopsies were fixed in 10% buffered formalin, routinely processed and paraffin embedded sections were taken and stained with Hematoxylin and Eosin. The stains were interpreted and statistical analysis of the data obtained was done. The most common specimen received was appendix which accounted for 49%, followed by cholecystectomies accounting to 38%, 5.5% biopsies from rectum and anal canal, 3%gastric mucosal biopsies, 3% biopsies from colon, 1% mucosal biopsies from small intestine, 0.5% mucosal biopsies from esophagus. In this study it was observed that appendix was the most common specimen received, followed by gall bladder. The most common tumor of esophagus was squamous cell carcinoma. The most common tumor of stomach was adenocarcinoma. Inflammatory lesions were more common in small intestine. Histopathological analysis of gastrointestinal lesions helps the clinicians for proper follow up and specific treatment. This study gives an overview of the various histopathological specimens received in the pathology department.