Cystatin C is a cysteine proteinase inhibitor belonging to type 2 cystatin superfamily produced endogenously. The main catabolic site of cystatin C is Kidneys. It has been shown that cystatin C is a better marker of kidney dysfunction compared to creatinine. We in the present study tried to evaluate the cystatin C and creatinine levels in normal and hypertensive subjects and correlate the cystatin C levels with blood pressure and kidney functions in this group of the population. Methods: This prospective cross-sectional study was conducted in the Department of Biochemistry and General Medicine, Rajiv Gandhi Institute of Medical Sciences, Adilabad. A total of 105 patients were included in this study 56 patients were allotted to cystatin C group for estimation of cystatin C and 49 patients were allotted to creatinine group for estimation of creatinine levels. The results were arranged as quintile of cystatin C (Q1 – Q5) subgroups based on levels in cystatin C and similarly quintile of creatinine (Q1-Q5) in creatinine group. The coefficient of correlation ‘r' values was estimated for cystatin C and creatinine with SBP and DBP. Results: Cystatin C levels of all the 5 sub-groups (Q1- Q5) the strong positive correlation was shown for SBP by Q4 with ‘r' values +5.48 and Q5 with ‘r' values +6.43 and DBP has positive correlation only in Q5 group value +0.44. Similarly, in the creatinine sub-groups, a positive correlation between SBP and creatinine levels found in Q4 and Q5 groups. The SBP in Q4 was with ‘r' value +0.31 and Q5 was with +0.49 the values of DBP did not show a significant positive correlation with creatinine levels in all the groups. Conclusion: it can be concluded that cystatin C is a better marker of kidney functions and can be used to evaluate the blood pressure changes affecting the kidneys. However, the cystatin C test is costly and may not be feasible in low resource settings. In such cases, the continuance of the use of creatinine may be done for monitoring the kidney functions in hypertension.