The study was conducted in five major Binasoybean-2 growing areas of Bangladesh, namely Noakhali, Laxmipur, Chandpur, Barisal, Comilla. The major shares of total cost were human labour, power tiller, fertilizer and irrigation. The average total cost of production in field level of Binasoybean-2 is Tk. 31382.71. The net returns were Tk. 39738.40, Tk. 32353.80, Tk. 31467.89, Tk. 30499.63 and Tk. 20688.88 ha-1 followed by Chandpur, Laxmipur, Noakhali, Barishal, Comilla, respectively. The highest net return (Tk. 39738.40 ha-1) was found at Chandpur district and the lowest net return (Tk. 20688.88 ha-1) comes from Comilla district for Binasoybean-2. The undiscounted benefit cost ratio over full cost basis were 2.29, 2.02, 2.01, 1.98 and 1.65 for Binasoybean-2 in field level for Chandpur, Noakhali, Laxmipur, Barishal and Comilla, respectively. The highest Benefit Cost Ratio (BCR) is 2.29 in Chandpur district and the lowest BCR is 1.65 in Comilla district. The highest yield gap between research station and field level was in Comilla (397.50 kg ha-1) and lowest in Chandpur (83.50 kg ha-1). The yield gap between research station and farm level in percentage were 18.30, 8.49, 11.48, 40.43 and 21.30 for Binasoybean-2 in Noakhali, Chandpur, Laxmipur, Comilla and Barishal, respectively. Binasoybean-2 growers faced various constraints to Binasoybean-2 cultivation like, disease and pest infestation, non-availability of quality seed and fertilizer at proper time, lack of soil moisture during sowing time, lack of credit facilities etc. Binasoybean-2 production in the study areas is profitable. Binasoybean-2 farmers received higher return on their investment