Public transportation is a service provided by the government in several major cities, whose purpose is related to reducing congestion. In addition to the jammed that underlies the existence of public transportation, the people's desire to have a safe, comfortable, easy and affordable transportation mode. The aims of this research are to analyze the relationship between service quality and passenger satisfaction in BRT Semarang. Survey questionnaire was conducted from 150 passengers, to analyze and test the relationship between variables using a simple regression test with the help of SPSS 22. The results obtained explain that three of the five dimensions of service quality positively contribute to passenger satisfaction in the Semarang BRT. Thus, this means that to increase customer satisfaction, the BRT Semarang must pay more attention and increase efforts into the five dimensions of service quality, especially the weakest of tangibility and assurance