Background: Urinary tract infection (UTI) is one of the most widely spread and costly medical complications of pregnancy, occurring in nearly 20% of all pregnancies. Aim: The purpose of this study was to estimate the prevalence of UTI among pregnant women and risk factors in Derna city during 2017 – 2018. Results: A total of 140 pregnant women were recruited in this study. The mean age of pregnant women was 28.24±5.6 ranges from 17 to 49 years old. The prevalence of urine tract infection was 49.3% (69 of 140), 66 (47.1 %) of them had urine tract infection symptoms. More than half of the women were in second trimester (53.6%), 70% were multi-gravid and 55% had previous history of urine tract infection. A total of six bacteria species were isolated and identified, Staphylococcus aureus was more frequently isolated (55.9%), following by E. Coli (17.6). Conclusion: Urine tract infection is commonly encountered in pregnant women, Regular antenatal care including routine urine testing at every visit to detect and treat asymptomatic bacteriuria is recommended. Patients should be taught proper urine sampling techniques of clean catch mid stream urine. Positive samples should be sent for sensitivity and appropriate antibiotic use according to tested sensitivities