A new reversed phase simple, economic and specific validated high performance liquid chromatography method for estimation of moxifloxacin in human plasma. Mobile phase used is acetonitrile: trisodium phosphate (5:95% v/v). UV detection at 294nm. The bioanalytical procedure involves de proteination of plasma with liquid-liquid extraction. The percentage of relative recovery and coefficient of variation of accuracy and precision were within acceptable limits. The method proved in simple, cost effective and sensitive for estimation of moxifloxicin in human plasma