Phyllanthus muellerianus is a large genus of the Euphobiaceae, is popularly known as ‘nvo nkwo’in Oghe traditional medicine. This species is used in the folk medicine for the treatment of sexually transmitted desieases. The aim of this study was to isolate and identify and spectroscopic characterize of sample GH3 from leaves of P. muellerianus. Sample GH3 from leaves of P. muellerianus was analyzed for their absorption, functional groups (CH, H, OH and NH) and 1H NMR resonance peaks (7.2 ppm and 6.7 ppm), resulting in the identification of benzopyran’s groups, suggesting 2H-1-benzopyran (chromene). The identification of benzopyran’s groups is here reported for the first time in P. muellerianus.