Healthcare-associated infections (HCAI) are a major setback for any organization. The most important mechanism of spread of these HCAI is via the contaminated hands of the healthcare givers that is doctors, nurses, other staff or relatives/friends of the patients. Contaminated environmental surfaces are another important reservoir for the spread of these infections. However, they are often under recognized. HCA is one of the most important public health problems in most countries of the world. In any healthcare setup basic infection control, measures can reduce the rates of healthcare-associated infections, which help to reduce the length of stay, nosocomial infection and cost. It is important to implement the quality system which can be executed and monitored by using compliance measures in the routine process of the hospital in order to improve the standard of patient health care. The quality of hospital infection control program is a reflection of the overall standard care provided by the institution. The current study is aimed to help in assessing the pre-existing knowledge regarding infection control practices among doctors and nurses. In our study, both doctors and nurses had a fair knowledge about the spread of nosocomial infections, practised safe patient-care protocols, and had positive attitudes. Still, some gaps have been identified in their knowledge and practice of infection control. It is suggested that an effective training sessions should be organized at the time of hire and in-service periodically which might help to decrease the healthcare associated infections.