Fluoroquinolones are being extensively used for the treatment of OPD patients suffering from various infective diseases. Extensive use of these of drugs has resulted in high drug resistance among common pathogens. We analyzed the current antibiotic resistance pattern of E.coli to fluoroquinolones for four consecutive years. 9186 samples were processed and 716 E.coli were isolated. Antimicrobial susceptibility testing was done in accordance with latest Clinical and Laboratory Standards Institute (CLSI) guidelines.6644were urinary samples followed by blood (1321), pus ((704), and miscellaneous (517). Maximum positivity was seen in pus samples (43.18%, 308/704) followed by miscellaneous (18.56%, 96/517), blood (18.01%, 238/1321).Antibiotic resistance trend, during 2016, revealed least resistance against levoflox (12%) followed by gatiflox (13%) and moxifloxacin (28%). Maximum resistance was seen against norflox (42%) and ciproflox (40%). Nitrofurantoin showed minimum resistance (8%) among all. We observed a pattern of decreasing resistance against fluoroquinolones for E.coli during the study period.