Chronic renal failure is one of the slowest progressive and irreversible diseases of kidney function which is characterized by low glomerular filtration (GRF). Chronic kidney diseases (CKD) is the 17thcause of disability and 12th major cause of death. Dialysis is the artificial way of carrying out removal of toxic metabolic products from blood, when the kidneys are not functioning. Even though there is a dramatic improvement of dialysis technology and pharmacological treatment, mortality rates for dialysis patients are still high. In India within a year approximately 9-13% hemodialysiate patients die. Highest cause of mortality among CKD is sudden cardiac death (SCD), in which death occurs from sudden unexpected cessation of cardiac activity with hemodynamic collapse. Serum creatinine and urea are considered as the most sensitive markers for the detection of the renal failure. In CKD patients one finds both hyperkalemia and hypokalemia which can be due to a decreased kidney function or due to diuretic administration eventhough their impact on mortality and end-stage renal disease (ESRD) is not very well understood. The present study was undertaken to evaluate the pre and post dialysis samples for values of renal biochemical markers like serum urea, creatinine and electrolytes. Total 68 CKD patients were observed. Statistically significant difference was observed between pre and post dialysis blood urea, serum creatinine, sodium and potassium levels. Hemodialysis is an effective and efficient process in removing these undesirable metabolites, helps increase life expectancy.