Background: Vitamin B12 deficiency has been a well known health problem. Recently, there are certain studies proving it more common as previously understood. Many have sorted for vegetarian food, with about 10% of the population deciding not to consume animal foods. This depends on ethical, ecological and religious belief, but also on health reasons. When the reasons are ethical, individuals ignore on nutritional value. The link between vitamin D and anemia has emerged in recent years, indicating potential roles for vitamin D in iron homeostasis and erythropoiesis. Very few studies exist on vitamin D levels in anemia. Studies are including vitamin D and vitamin B12 are significantly few. Hence, the present this study aims to find the trend of vitamin D levels in vegetarians having vitamin B12 deficiency. Methods: All clinical and lab data of cases from Jan 2017 to May 2018 from the lab in which Vitamin B12 assay has been done was obtained from lab records. The vitamin B12 assay was done on immunoassay analyzer Roche e411 with chemilumeiscence method. Vitamin B12 levels <180ng/dl was considered deficient. The same was again grouped based on Vitamin D levels as deficient and insufficient. Results: The percentage of subjects in deficient group with low vitamin B12 levels had decreased haemoglobin <11 was 35% when compared with sufficient of 9.4%, P value = 0.02 was significantly correlating. Similarly the MCV(>97) and MCHC(<33) had higher percentage in insufficient Vit D3<30 group with 47.3% and 56.3% respectively. Both parameters also have significant P value being < 0.05. Conclusion: The study found that apart from vitamin B12 deficiency, there are higher chances of these patients being associated with vitamin D3 deficiency.