Fungal infections from the Candida have significant cause of blood stream infection. This is troublesome among those who have been hospitalized with serious underlying diseases or those who are immunocompromised. To know the prevalence and types of Candida species in blood stream infection and their antifungal susceptibility pattern. The study was carried out in the Department of Microbiology, PDU Medical College, Rajkot from September 2015 to August 2016. Total number of samples are 207. Blood culture specimens were collected and processed for, culture on SDA and HCDA, Slide culture, Gram stain, Germ tube. Antifungal susceptibility testing was performed by modified Kirby Bauer method as per the CLSI guidelines. 58 (28.01%) Candida spp. [C. tropicalis (18), C. glabrata(14), C. gullermondii(12), C. parapsilosis(10), C. albicans(4)] were isolated from 207 specimens. Out of these, 203 (98.06%) from NICU/PICU and 4 (1.93%) from Skin ward, predominantly Males (57.97%). The isolates sensitivite to Voriconazole (100%), Fluconazole (98.88%), followed by Ketoconazole (73.03%) and Clotrimazole (68.62%). Maximum resistance observed to Amphotericin B, Nystatin, Miconazole, Itraconazole. Candidemia is major cause of mortality due to lack of antifungal therapy. Blood stream infections by Candida species have shown highest rates of inappropriate therapy among all BSIs. Strategies are needed to rapidly identify cases of candidemia who are already suffering from serious underlying disease and develop rapid diagnostic technology that widely available and cost effective. By knowing Antifungal susceptibility pattern, patients who are at increased risk for developing nosocomial candidemia should be treated early with empiric therapy that reduced unnecessary patient mortality.