Aim: To compare and measure molar anchorage loss during en-masse retraction using mini-implant and conventional mechanics. Objectives: 1) To evaluate the molar anchorage loss during en-masse retraction of anterior teeth by using conventional mechanics. 2) To evaluate the molar anchorage loss during en-masse retraction of anterior teeth by using the orthodontic mini-implant as absolute anchorage. 3) To measure and compare the molar anchorage loss during en-masse retraction using mini-implant and conventional mechanics. Methods: The study was carried out on 10 patients requiring extraction of four first premolars and maximum retraction. On the right side (experimental side) mini-implants were placed between the second premolar and first molar and an e-chain was engaged from the head of the implant to the crimpable hook. On the left side (control side) an e-chain is given from crimpable hook to molar hook for en masse retraction. The retraction space closure was calculated using vernier caliper on the study model after 28weeks of retraction. Lateral cephalogram was taken before and after orthodontic retraction for calculating molar anchor loss. Results: The retraction space closure after 28 weeks showed a mean value of 3.59+0.68mm on the mini-implant side and 3.98+0.62mm on the conventional mechanics side, which was statistically significant. The anchor loss of 0.17+0.3 mm was observed on the mini-implant side and 1.32+0.62 mm was observed in the conventional mechanics after retraction, which was found to be statistically significant. Conclusion: In this study, the molar anchor loss of 0.17+ 0.3mm was seen on the mini-implant side and 1.32+0.6mm on the conventional mechanics side which was significantly more by 1.15mm than the mini-implant side. The closure of extraction space on the mini-implant side was done by retraction of anterior teeth and minimal anchorage loss, while in the conventional mechanics side there was anchor loss of anterior as well as posterior teeth.