Guiera senegalensis generally occurs as a shrub or a small tree which belongs to the Combretaceae family and locally named in Sudan as “Gabeish”. It has medicinal properties such as anti-inflammatory, anti-oxidant, anti-asthmatic, potential anticancer, anti-anaphylactic and anti-microbial activities. The plant was collected from the traditional market in Omdurman. Prepared ethanolic extract of leaves were used to evaluate the phytochemical screening to detect the presence of flavonoids, alkaloids, tannins, phenol, saponins, carbohydrate,cumarins and absence of anthraquinones and triterpenes. The phytochemical contents revealed tannins (1.662), alkaloids (3.524), flavonoids (39.350) and biological study of leaves extract showed activity against Escherichia coli, Staphylococcus aureus, Bacillus subtile, Papillus subtile and Candida albicana.