Fomite is an inanimate object or substance that could serve as a vehicle for transmission of infectious organisms amongst individuals. A large number of factors may affect the contamination rate of fomite such as moisture, consistent use and overall cleanliness. Fomites that are found in public places, restaurants, hotels, hospitals and restrooms may include among others handbags, mobile phones, money, door handles or knobs of showers, conveniences, faucets and toilet seats, chairs, lockers, sink and tables. Ladies handbags are multipurpose personal gadgets which may usually habour several kinds of microorganisms such as bacteria. The presence of viable pathogenic bacteria on inanimate entities had been reported by earlier investigators. In this study, a total of one hundred (100) handbags from female undergraduate Microbiology students of Gombe State University (GSU), were investigated for bacterial contaminants and their resistance or otherwise to some commercial antibiotics using standard Microbiology procedures. The results obtained revealed the presence of six (6) different bacterial species, namely Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus subtilis. The antibiotic sensitivity test showed that all the six (6) bacteria were sensitive to Pefloxacin while five isolates (S. aureus, E. coli, P. mirabilis, K. pneumoniae and Bacillus subtilis) , two isolates (B. subtilis and P. mirabilis) and another two isolates (K. pneumoniae and E. coli) were sensitive to chloramphenicol, amoxicillin and streptomycin, respectively. In conclusion, high level of bacterial contaminants were observed from the studied handbags and found some of the contaminants resistant to the tested antibiotics hence, appropriate use of effective disinfectants is highly encouraged to reduce the magnitude of bacterial contaminants and likelihood of transmitting drug recalcitrant organisms.