Burns are one of the most common and devastating forms of trauma. Patients with serious thermal injury require immediate specialized care in order to minimize morbidity and mortality. Data from the National Center for Injury Prevention and Control in the United States show that approximately 2 million fires are reported each year which result in 1.2 million people with burn injuries. The present study, “Bacteriological profile of burn wound infections” was carried out in Department of Microbiology, in a tertiary care hospital, over a period of one year from Jan 2016 – Dec 2016. A total of 50 patients of all age groups and both sexes admitted in our Burn Care Unit were selected for this study. In the present study, Majority of patients were in the age group 31-40years. Out of 50 patients studied, 28 patients were male and 22 patients were females. A total of 128 organisms were isolated and Pseudomonas aeruginosa (44 cases) was the most common isolate followed by Staphylococcus aureus (37 cases) and coagulase negative staphylococci (29 cases). The other isolates included Klebsiella pneumonia, Escherichia coli, Proteus mirabilis, Citrobacter freundii and Enterococcus species. Modern infection control practice has been effective in reducing or eliminating endemic pathogenic and/or antibiotic-resistant organisms, preventing the establishment of newly introduced pathogenic and/or antibiotic-resistant organisms as the predominant nosocomial flora of the burn unit, and preventing reseeding of such strains back into the burn unit from patients housed in the adjacent convalescent ward.