Evolution of Wireless Body Area Network (WBAN) in telemedicine field keep increased along with needs and enhancement of services, nevertheless an efficient and effective form of communication becomes essential for any type of wireless communication network. One of the most important parameter for designing communication network is path loss exponent. Based on that, the purpose of this research is to know the propagation characteristics for on-body and off-body communication by comparing the simulation and measurement using magnetic textile antenna at 2.45 GHz and 924 MHz with distance variation in LOS and NLOS conditions. In this research, friis formula is used to get the path loss exponent value that shows the characteristics of propagation. The calculation of path loss exponent value for simulation and measurement for on-body communication with LOS and NLOS conditions are between 1.7-1.9 while off-body with LOS and NLOS conditions are between 2.1-5.2. These results show that the body affects path loss exponents value and have own characteristics.