The aim of this study was to determine the parameters of acute toxicity of venom of the kind Tetraponera, on the rats. It was an experimental prospective study carried out at the traditional phytotherapy center of treatment of “ASMASIDAP”, during 5 months, from 1st Feburary to June 30, 2015 and in the laboratory of the FMSP of the University of Douala. This center is situated in Ebang-Mekong, Bikok subdivisionin and Méfou and Akono division; the sampling consisted of a group of 42 rats distributed in 6 batches plus one witness. The lethal dose 50 obtained by the graphical method of Muller and Tainter gave us a value of 653.5 ± 0.12 mg / kg of body weight; The rats, after receiving the injections, hugged each other, squirmed, squealed, their heartbeats seemed to speed up. This study determined the LD50, which, according to scale of Hodge and Sterner, would be slightlytoxic. This study permits us to determine the acute toxicity of venom of kind of tetraponra. LD50 = 653.50mg/Kg of body weight.