Lassa fever is a disease of public health importance with high mortality and morbidity especially among rural dwellers.This study was done to determine the knowledge of transmission, risk factors, prevention, symptoms and the preventive practices against Lassa fever among adults in Ukpom, a rural community in Akwa Ibom State, Nigeria. A community based descriptive cross-sectional study was done among 228 adults selected by multi-stage sampling in 2016 using a pre-tested interviewer-administered questionnaire and analysed with SPSS.Ninety point eight percent (90.8%) claimed to have heard of Lassa fever mainly through radio and TV (64.9%). The majority knew that consuming food contaminated with rat feces and urine (88.6%) including eating poorly cooked rats (82.5%) transmits Lassa fever. Most knew that a clean environment (95.2%), proper covering of food (93.4%), no holes in homes (93.4%) and not eating rats (89.5%) prevents Lassa fever .The top 2 risk factors for Lassa fever known were non- covering of food (91.7%) and poor refuse disposal (89.9%). Common symptoms known were headache (69.3%), fever unresponsive to antibiotics (68.4%) and vomiting (66.2%). Major practices to prevent it were covering of food (98.2%), not eating rats (97.4%) and keeping a clean environment (95.6%). Only 15.8% stopped burning bushes, 32.9% stopped drinking garri and 17.5% fumigated their houses as practices to prevent Lassa fever. Age was significantly associated with level of knowledge. (p=0.000). Good level of knowledge of Lassa fever was found among community members though some misconceptions still existed. Intensified health education is recommended.