Staphylococcus aureus is a common hospital and community-acquired pathogen known to be frequently associated with wound infections. Therefore, the aim of the research was to determine the incidence of Staphylococcus aureus in patients with wound infection at University of Port Harcourt Teaching Hospital, Rivers State, Nigeria. A total of 150 specimens from different types of wounds (traumatic wound, caesarean section, scrotal wound, surgical wounds, burns, diabetic foot, and plastic surgery) were collected from the Hospital for a period of six months and processed for isolation of S. aureus, following standard microbiological procedures. Samples were cultured on sterile mannitol salt agar (MSA) plates and characterized phenotypically based on cultural and biochemical identities. Data obtained showed 38.7% of the wound cases were contaminated with Staphylococcus aureus isolates. It was observed that the organism mainly occurred in burns (52.63%), while the lowest incidence was related to plastic surgery (18.2%) contamination. The study showed that 10.3% of isolates were associated with biofilm formation, while gamma hemolysis and beta-hemolysis was observed in 93.1% and 6.9% of isolates, respectively. The results of this study represent serious public health concerns, thus emphasizing the need for proper wound management.