The research aimed to measure HbA1C in normal Sudanese non-diabetic pregnant women attending antenatal care at Ribat University Hospital, Khartoum state during the period from Sep 2017 to Nov 2017, and to explore the effect of pregnancy on HbA1C. A Cross sectional facility based study was conducted in The National Ribat University hospital during the period from Sep 2017 to Nov 2017 among the Normal Sudanese pregnant, non-diabetic females of ages between 18-40 years. Twenty women were included as a pilot, ten of them at first trimester and ten at third trimester. Correlations between the variables were estimated and P value less than 0.05 was considered statistically significant and used to determine the relationship between the Multiple factors of pregnancy and HbA1c level. The mean value of HbA1c at first trimester of pregnancy was found to be 4.7% with a range of (3.5% - 5.5 %) and the mean value of HbA1c at third trimester of pregnancy was found to be 3.98% with a range of (2.8% - 5.4%). The mean value of HbA1c in normal pregnancy was found to be 4.37% with a range of (2.8% - 5.5 %). There was no correlation between normal FBG, Hb level, daily caloric intake, age, PH. of DM, PH. of GDM, family history of DM and the level of HbA1c. According to this study the mean value of HbA1c in Sudanese healthy pregnant women is 4.37 found within the normal Sudanese values of HbA1c. HbA1c is lower in the third trimester compared to first trimester.