The aims of the present investigation were to evaluate (1) HIV/AIDS related knowledge, attitudes and risk perception among the physiotherapist in Chennai, Tamilnadu, India (2) to identify predictors of willingness to provide care for patients with HIV infection. A cross sectional survey was done among 300 physiotherapists working in different hospitals, physiotherapy clinics and institutions offering physiotherapy services in Chennai, Tamilnadu, India. The internal constancy for HIV/AIDS related knowledge was found to be 0.717. The attitudes and risk perception among the participated physiotherapists was found to be 0.723. The results indicated that HIV/AIDS related knowledge, attitudes and risk perception among the physiotherapist in Chennai Tamilnadu, India was acceptable. From the present investigation it was also found that there was significant association between physiotherapist’s attitude towards HIV/AIDS and their willingness to treat HIV/AIDS patients.