Hepatitis B infection is caused by hepatitis B virus (HBV), an enveloped DNA virus belonging to family Hepadnaviridae that infects the liver and causes hepatocellular necrosis and inflammation which are serious public health problem worldwide. The Present study was conducted in the department of microbiology on 456 female patients. The serums were obtained by centrifugation & test was done. The Hepatitis B surface Ag was detected by using HEPACARDS kit. In our current study on 456 female patients were screened for HBsAg and we found that 5.3% female patients were reactive with HBsAg in serum and 94.7% were Non reactive with HBsAg out of 456 female patients. Higher prevalence of HBsAg was reported in most sexually active age group.i.e.21-40 years (57.5%) and least in age group 81-100 (0.4%). In the light of current study, it can be concluded that the Hepatitis B contributes significantly to chronic liver diseases in India and being transmitted through parenteral, sexual or perinatal mode. The study revealed high prevalence rate of HBV among female patients (5.3%).