Background: Acute viral hepatitis (AVH) is a major public health problem and is an important cause of morbidity and mortality. Acute Viral Hepatitis A infection among adults .in developing country is low due to pre exposure of Hepatitis A virus (HAV) during childhood and adolescence. Anti-HAV prevalence in population worldwide has grouped countries into high, intermediate low and very low endemicity. The anti-HAV prevalence in Indian population is of high endemicity. But, there is heterogeneous exposure of hepatitis A virus in different region of the country. In India the age of acquiring HAV has a world-wide distribution and affects infant and young children in developing countries and its epidemics are not very common. HEV is restricted to tropical countries and affects older children and young adults and its epidemics are common. Studies suggested that HEV is etiologically responsible for 10%–95% of admitted cases of hepatitis. Exposure rates over a period of time are different in different parts of the country and in different socioeconomic groups. Objective: To determine the prevalence of Hepatitis A Virus (HAV) and Hepatitis E virus (HEV) infection among patients of suspected acute viral hepatitis admitted in Sola Civil Hospital, Ahmadabad / in a tertiary care hospital, Ahmadabad, Gujarat. To determine any change in the epidemiology of these infections with the age, gender and seasonal trends. Methods: This study was conducted in the Department of Microbiology, GMERS Medical College & Hospital, and Sola, which is a leading Tertiary care hospital and the major referral centre of Ahmedabad, Gujarat.