This complete enumeration, cross-sectional comparative study was conducted at Rajiv Gandhi Medical College in Kalwa, Thane, Maharashtra state, India. After obtaining permissions from the Institutional Ethics Committee and institutional authorities for conducting the study, the purpose of the study and the procedure for objective structured practical examination (including check-list based marking system) was explained to first-year MBBS students and written informed consent was obtained from those willing to participate in the study. Traditional practical examination was first conducted and overall marks (out of 5) were allotted by the examiners. In the objective structured practical examination, the examiners were provided with a pre-validated checklist and the maximum score obtainable was 5 marks. A total of 54 first-year MBBS students (27 females and 27 males) participated in the study. The students obtained significantly higher scores in traditional practical examination but the gender differences in scores were not significant.