The aim of our study is to assess the frequency of urinary tract infections in clean catch midstream urine, to isolate the urinary tract infection causing organism and their anti microbial sensitivity to various antibiotics in chronic renal failure patients undergoing hemodialysis. Our study was carried out in the time span of 12 months from January 2018 to December 2018 at GMERS Medical College Gandhinagar, Gujarat. The study included 1225 patients of all age group who had clinically evident chronic renal failure and taking hemodialysis. Out of 1225 patients presented clinically as chronic renal failure and taking hemodialysis, 365(29.80%) patients were culture positive and 860(70.20%) patients were culture negative. Out of 1225 patients of CRF taking hemodialysis 891(72.73%) were male and 334(27.27%) were female. In the present study maximum number of patients 97 were from age group 61-70 years followed by 57 patients from 21-30 years age group. Out of 365 cultures positive isolates 301(82.47%) were gram negative isolates and 64(17.53%) were gram positive isolates. Both gram negative and gram positive isolates are 100% sensitive to imipenam and least sensitive to amoxicillin 15.28% and 10.94% respectively. MDR in gram negative isolates were 19.27% and in gram positive isolates were 12.69%. our study is helpful in need for continous evaluation of local antibiotics sensitivity patterns for the formulation of rational antibiotic policy which will reduces further spread of antimicrobial resistance.