Background & Objective: Hepatitis B and Hepatitis C are significant problems in the management of haemodialysis patients. We aimed to investigate the incidence and prevalence of HBV and HCV infection in the hemodialysis patients as well as risk factors for infection. Methodology: All adult patients receiving maintenance hemodialysis (n=217) in General hospital, gandhinagar were studied between September to December 2018. Testing for Hepatitis B surface antigen (HBsAg) and anti-HCV antibodies was performed at Department of Microbiology, GMERS medical college, Gandhinagar. Results: The prevalence and incidence rates were 2.30% in HBV patients and 5.06 in HCV patients respectively. There was a significant correlation of the prevalence and seroconversion of HCV and HBV with number of blood transfusion and duration of heamodialysis. Conclusion: Patients on maintenance hemodialysis have lower incidence and prevalence of HCV infection and lower rates of HBV infection in this study.