Background: Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important arthropod borne viral diseases. Dengue is emerging as major public health concern in India and spreading with increased morbidity. Objective: This study was carried out to determine the seroprevalence of Dengue infection among patients attending in Gandhinagar civil hospital attached with medical college during the period of January 2018 to December 2018. Methods: A total of 2545 blood samples received in the department of microbiology, were tested for the confirmation of suspected cases of dengue. Dengue NS1 antigen and Dengue IgM antibody ELISA tests was performed for the confirmation of dengue cases. We estimated the incidence by applying age, sex and season adjusted dengue positivity. Results: Out of 2545 samples tested, 639were positive for dengue infection, among the positive cases 503 were NS1 antigen ELISA positive and136 were IgM ELISA positive. Higher incidence in male patients was noted. Number of positive cases was increased in rainy season. Conclusion: Virus activity is high during monsoon and post monsoon period which coincides with increased vector breeding. This study thus emphasizes the need for continuous sero epidemiological surveillance for the timely formulation and implementation of effective dengue control programme.