A study was conducted for the isolation and identification of soil micro-flora in rhizosphere of B. racemosa (L.) Spreng and R. serpentine (L.) Benth. ex Kurz, trees from Western Ghats region of Uttar Kannada, Karnataka, India. Soil samples were collected from rhizosphere of B. racemosa and R. serpentine plants, during the months of February 2022. Soil microbes were isolated by using soil dilution technique. The total number of bacteria isolated from B. racemose and R. serpentinais rhizosphere is 2,666,600 and 4,461,160 per gram of soil respectively. The rhizospheric fungal isolates present in B. racemosa and R. serpentina plants is 3,281,200 and 1,946,200 per gram of soil respectively. In B. racemosa rhizosphere, the percentage contribution is Penicillium sp with 13.679%, In R. serpentine rhizosphere, the highest percentage contribution is Cladosporium sp., Trichoderma sp.with17.663% and 17.391% respectively. In rhizosphere of B. racemose, 19 different species belonging to 15 genera were isolated; among which Penicillium species is dominating over other fungal species isolated. In rhizosphere of R. serpentina, 18 different species belonging to 11 genera were observed; among which Trichoderma sp.is dominating over other fugal species isolates. The most frequently isolated fungi from the rhizosperes of B. racemosa and R. serpentine plants are Penicillium sp and Trichoderma sp.For the first time the rhizospheric micro-flora i.e., bacteria and fungi was reported from B. racemose soil sample collected from Western Ghats region of Uttara Kannada. For the first time the rhizospheric myco-flora was reported from R. serpentine soil samples collected from Western Ghats region of Uttara Kannada. All the bacteria and fungi isolated was new report to Wester Ghats region of Uttar Kannada, Karnataka, India.