The year 2009 witnessed emergence of novel H1N1 subtype of influenza A virus. This novel strain rapidly spread across the world and caused pandemic. In 2010, WHO declared pandemic to have crossed its peak and also warned that it will continue as seasonal influenza outbreaks in winter seasons. Several aspects of the epidemiology of influenza A H1N1/2009 strain have not been accurately determined. We sought to study whether the age & gender affects susceptibility to influenza. During the study period of one month, patients categorised as category C under national influenza guidelines and admitted to isolation ward were screened for influenza. Real-time reverse transcriptase PCR (rRT-PCR) was used for detection of influenza viral RNA. For PCR setup, WHO & CDC guidelines were followed. The result given was either Influenza A/H1N1/2009pdm strain positive or negative. Out of 75 samples received in the laboratory, 32 samples were positive (positivity rate of 42.66%). Positivity rate for females (46.51%) was higher than males (37.50%). Positivity rate was maximum in 70-79 years age group (100%) followed by 30-39 years age group (46.15%) and 20-29 years age group (44.44%). None of the patient was positive in 10-19 years age group (Positivity rate of 0%). The influenza (H1N1/2009) virus is still active several years after the 2009 pandemic in the various parts of the world. The incidence of H1N1 influenza is higher in old individuals. This emphasizes importance of vaccination especially in high risk group before the start of the flu season.