Hydatidosis is a parasitic disease caused by the cestode Echinococcus granulosus. The present study focused on the multi-diagnosis of a clinical case including; histopathology, the clinical presentation of the patient, and the molecular diagnosis of the tissue. This is a cross-sectional study for patients diagnosed with hydatid cyst disease. Four hospitals in Gaza Strip were included as follows: European Gaza Hospital, AL-Shifa Hospital, Al-Ahli Arab Hospital, and Nasser Hospital (Histopathology Department). A total of 15 clinical cases of hydatidosis are described, with hydatid cysts collected from the four hospitals diagnosed by the clinical presentation of each case, histopathology, and molecular diagnosis. A total of 46.7% of the examined hydatid cyst disease cases were from the liver. The sequencing and analysis revealed one genotype of E. granulosus (G1) responsible for these human hydatid cysts. It is concluded that hydatid cyst disease occurrence is confirmed in the examined human tissue samples and belongs to genotype G1. It is recommended that hydatisosis is known among senior surgeons, we recommend that these cases should be presented in front of early career surgeons, interns and Internists in Gaza Strip Hospitals.