Introduction: Pseudomonas aeruginosa has been emerged as an important opportunistic pathogen. Being an extremely adaptable organism, it can survive and multiply even with minimal nutrients and is one of the leading causes of hospital acquired infections. P. aeruginosa exhibits intrinsic resistance to several antimicrobial agents. As a result of indiscriminate use of antibiotics, the spread of multidrug resistance (MDR) is now a global problem. Its general resistance is due to a combination of factor.Emergence of carbapenem resistance mainly Metallo-Beta-Lactamase (MBLs) in Pseudomonas aeruginosa which is considered as a world wide public health concern. Objectives: To study the detection of Pseudomonas aeruginosa and its antibiotic susceptibility pattern from various clinical samples in Tertiary Care Hospital, Bhavnagar, Gujarat. Materials and Methods: The Present study was undertaken at Microbiology Laboratory, Sir T. Hospital, Bhavnagar. 300 isolates of Pseudomonas aeruginosa were collected from various clinical samples between November-2013 to August-2014 .They were subjected to antibiotic sensitivity testing by Modified Kirby Bauer Disc Diffusion Method as per CLSI guidelines. Quality control of the test was done by standards ATCC strain P. aeruginosa 27853. Results: 300 Isolates were included in the study, out of which 95(32%) showed Imipenem Resistant. and were 100% resistant to Cefoatxime, and Imipenem. Gentamicin and Ciprofloxacin showed 84% and 93% resistance. Amikacin and Ofloxacin showed 89% resistance each. where as Piperacillin showed 75% resistance. Conclusions: Early detection will go a long way in making adjustments in empirical antimicrobial therapy. The study was coducted to formulate antibiotic policy and plan a proper hospital infection control strategy to prevent the spread of these MDR strains.