Diabetic foot infection is one of the most common complications of Diabetes and it is a major public health problem that leads to amputation if not treated. Moreover screening of the ulcers for microbial growth and antibiotic susceptibility will enable to initiate the appropriate antibiotic therapy. The present study was carried out to identify the profile bacterial pathogens from the diabetic foot ulcers and also to determine it’s antibiotic susceptibility pattern. Ninety four patients with diabetic foot ulcer attending Surgery outpatient department were included; demographic and clinical examinations was done by the surgeons and the ulcers were assessed as per the Wagner classification of ulcers. Wound swabs and pus were collected from the diabetic foot infections and were processed using standard Microbiological techniques. The results revealed Pseudomonas aeruginosa predominated followed by Klebsiella pneumoniae and Escherichia coli among negative groups whereas Staphylococcus aureus dominated [two strains were Methicillin Resistant Staphylococcus aureus (MRSA)], followed by Enterococcus spp and Streptococcus among Gram positive. The Gram positive bacteria showed resistance to cephalosporins, aminoglycosides and other first line drugs. ESBL production was observed maximum in Klebsiella spp followed by Proteus spp and Escherichia coli. Metallo β lactamases production for Pseudomonas spp was found to be positive among 12 isolates. Hence by performing culture and sensitivity of diabetic foot ulcers will enable the antibiotic sensitivity pattern which will be helpful in determining the drugs for the empirical treatment thereby preventing indiscriminate use of broad spectrum antibiotics.