Background: Hepatitis E is an important public-health concern as a major cause of enterically transmitted hepatitis worldwide, spread primarily by faecal contaminated drinking water and is responsible for over 50% of cases of acute viral hepatitis in endemic countries. Aims & Objectives: To determine incidence of hepatitis E in jaundice patients admitted to the Tertiary care hospital, Jamnagar. 1) To assess Prevalence of Hepatitis E in various Socio-economic strata. 2) To determine various age and sex specific prevalence of Hepatitis E. Material & Methods: Total 377 blood samples of patient suspected of acute viral hepatitis were collected and tested for anti HEV IgM antibody by ELISA method (RecombiLISA). Observation and Result: Out of 377 patients, 48 patients (12.8%) showed anti HEV IgM antibodies. The anti HEV IgM antibodies were more in the 14-40 years (87.5%). Hepatitis E virus IgM antibodies positivity rate was more common in Males compare to females. Conclusion: HEV infection is maximum in young adults (14-40 years) than in pediatric and old age groups. Males (79%) are more seen to be infected with HEV than females (21%). In this present study there is no evidence of HEV in children's. Prevalence of HEV cases is mostly found in area of contamination of water.