Background: Documentation of the nursing method is crucial, however regularly ignored as a part of medical documentation. Paper-primarily based structures have been added to help the nursing documentation process. Objectives: The study aims to assess the effect of training program on knowledge and practice of nurses regarding nursing documentation. Methods: Quasi-experimental study was carried out in Omdurman military hospital extended from February 2019 to August 2019. A questionnaire and evaluation checklist was used to collect data from (203) nurses. They were selected by non-probability sampling technique (purposive sampling). The data were analyzed by computer software program (SPPS) version 20. Results: The study showed that more than half (60.6%) of nurses were female, (65%) had bachelor's degrees, (42.9%)had 1-2 years of experience and more than a third (34.0%) worked in general word. The nurse’s knowledge regarding the type of record system, pretest (51.7%) had unsatisfied knowledge. There was an increase in knowledge of nurses regarding documentation post-implementation of the program. Posttest (75.9%) with a highly significant relationship between nurses training courses and knowledge about the concept of quality in nursing (0.002) Study showed that the nurse's knowledge about nursing record forms was improved post educational program (48.3%) were satisfied knowledge compared with the pretest (69.05) were poor knowledge. Common nursing report errors were the change of shift and incidence report (73.4 %, 87.2%) in pretest which decrease to (50.2%, 44.3%) in the posttest, respectively. while the common nursing record error pretest Illegible record (69.3%), change in Patient condition (66.5%), Round book (73.9), Medication record error (60.1%). Conclusion & Recommendation: The teaching program had a positive effect on the quality of nursing care. The study recommended updating nurses’ knowledge and skills about documentation guidelines through continuous professional development.