Background: The study titled ―A cross-sectional analysis of Seropositivity of Hepatitis B & Hepatitis C amongst blood volunteer donors at tertiary health care centre: 10 year study. Method: Records of blood donors collected in the past decade were evaluated and information regarding screening tests was being noted. In addition, a questionnaire was being prepared to collected information about all subjects such as general profile, history of exposure to risky procedures or behaviours, family history of Hepatitis B infection, immunization against Hepatitis B in three doses etc. Result: The above table shows distribution of donors according to type of donor. 54028 (90.81%) donors were Replacement Donors while only 5467 (9.19%) donors were Voluntary Donors. Out of the total 59495 donors in past 10 years, 1020 (1.71%) were diagnosed to be HBsAg positive. Out of the total 59495 donors in past 10 years, 475 (0.79%) were diagnosed to be HCV positive. 74 (1.35%) of voluntary donors in last 10 years came out to be Hepatitis B positive. 58 (1.06%) of voluntary donors in last 10 years came out to be Hepatitis C positive. The above table shows the year-wise number of total donors and voluntary donors. It also shows the number of HBsAg. As per the above tables, there was significantly higher seropositivity among replacement donors as compared to voluntary donors. Conclusion: A large proportion of HBsAg and HCV infected individuals are not aware of their infective status. It is not only dangerous to not know this as these infections are also sexually transmitted to the spouse or any other sexual partners of such individuals. Also, at the time of emergency for the need of blood, such individuals may be a false assurance of source of blood until being tested and refused by the blood bank before actual donation. This may aggravate the situation by rendering in need to search for another source of blood. An increased public health education on the subject is necessary especially for those at a higher risk. Also, voluntary blood donation should be encouraged to fetch safe and tested blood in contrast to the replacement donation where transfusion-transmitted infections tend to be much higher.