Abstract: The arterial supply of the interventricular septum was studied by macroscopic and radiological examination after injecting radio-opaque material in 50 human hearts. During macroscopic study many intercoronary anastomoses were found in different regions of heart though they were short in length, small in caliber and thin walled. At radiological examination, the small septal arteries arising from the posterior interventricular artery and large septal arteries from the anterior interventricular artery were observed. The secondary branches from the main septal arteries displayed diverse patterns on the right and left sides of the septum. During radiological study, it was also recognized that the short septal rami from the posterior descending artery were distributed over the posterior or inferior one-third of the septum except its apical zone while the anterior descending artery through its septal branches supplied the ventral two-thirds of the septum and whole of the apical portion. Straight intercoronary anastomotic vessels were clearly discernible within the interventricular septum during radiological examination.