The property of iron to get reversibly oxidized and reduced is essential for its metabolic functions. Severe iron deficiency can produce cardiovascular disease. Wide range of biochemical markers are implicated in the pathogenesis of anaemia like Serum Ferritin, Malondialdehyde and Homocysteine. The purpose of the study is to assess serum ferritin, homocysteine and plasma malondialdehyde levels in Iron deficiency anaemic patients and to find whether anaemic patients are prone to cardiovascular disease or not. A case control study was carried out at Lata Mangeshkar hospital, Nagpur in which serum malondialdehyde and homocysteine levels measured in 30 diagnosed patients of Iron deficiency anaemia in the age from 19 to 40 years were compared with healthy controls. Malondialdehyde was estimated by the method of Randox laboratory. Serum homocysteine was measured by using ELISA kits. The data was analysed for correlation between levels of homocysteine and malondialdehyde with serum ferritin in patients of Iron deficiency anaemia. Serum malondialdehyde levels in patients with Iron deficiency anaemia were higher i.e. 0.680+0.349 (ng/ml) than in controls which were 0.144+0.102 (ng/ml) with a statistically significant value of p <0.05.Statistically, there was also a significant rise in homocysteine levels in patients with Iron deficiency anaemia i.e.76.105+16.836(ng/ml) as compared to 27.57+12.11 (ng/ml) in controls. An inverse relation exists between serum ferritin and markers of oxidative stress. Iron deficiency anaemic patients have elevated levels of malondialdehyde and homocysteine than the control group and both these parameters may be common mediators in the pathogenesis of accelerated atherosclerosis.