Drinking water has always been a major issue in many countries, especially in developing countries like Nigeria. The study was aimed to assess the effect of anthropogenic activities on water quality parameters at Gundutse River in Kura Kano, Northern Nigeria for a period of 3 month (April - June, 2019). Surface water sample for the study were collected from three (3) different sampling (A, B and C) sites based on the depth of the water and analysis of water was conducted either in situ or transported to the laboratory for further analysis. The physicochemical and microbiological properties of the water were determined using standard method. The result of physicochemical properties of the water showed that the temperature, pH, turbidity and electric conductivity ranges from 25 to 260C, 7.3 to 7.7, 140 to 155 NTU and 617 to 628μs/cm respectively. The average values of nitrate, phosphate, Dissolve Oxygen and Biochemical Oxygen Demand (BOD) of the water ranges from 1.80 to 2.13 mg/L 1.0 to 1.16 mg/L, 3.73 mg/L to 4.10 mg/L, 1.96 to 2.23 mg/L respectively. The result of microbiological analysis of the River showed that Escherichia coli, Salmonella typhi, Proteus mirabilis, Enterobacter sp. and Klebsiella pneumoniae were identified. The use of the river water for drinking may be hazardous. The study therefore, stresses on the need to control the faecal pollution of the water before use.