Background: The COVID 19 Virus globally has caused tremendous morbidity and mortality among the general population around the world. To assess knowledge, there has been limited number of studies done to know the prevalence of COVID-19 vaccine acceptance among pregnant and lactating women. Aims: To assess knowledge, attitude and acceptance of Covid-19 vaccine among pregnant & lactating females in rural area of western U.P. Methodology: This was a cross-sectional study conducted on pregnant & breast- feeding women attending the antenatal & postnatal OPDs in the department of Obstetrics and gynecology & department of Pediatrics at Teer thanker Mahaveer Medical college & Research center. A total 1560 patient responses were taken from age group 18 to 42 years. All were provided with a pre-validated questionnaire consists of multiple choice. These questions evaluate knowledge of COVID-19 vaccine authorization and published materials. The universal acceptance of the COVID-19 vaccine. Three- and five-point Likert scales were used to rate the responses. Results: knowledge regarding COVID-19 vaccination among participants was obtained from government agencies was insignificant given by 16%, somewhat significant by 40% and significant by 44%, from news channel and radio was 20%, 42%and 38%, from social media in 25%, 48% and 27%, from friends and family in 18%, 50% and 32% and through healthcare provider in 14%, 36% and 50% respectively. In response Strongly disagree, disagree, neither agree nor disagree, agree, and strongly agree were seen as responses to desire to take the COVID-19 vaccine seen in 3%, 4%, 12%, 26% and 55%, through obtaining natural immunity COVID-19 vaccine was seen in 18%, 25%, 22%, 18% and 17%, desire to spend money for the COVID-19 vaccine was seen in 4%, 12%, 14%, 35% and 35% and there was tendency to recommend vaccines to people who were already familiar with them 3%, 8%, 12%, 34% and 43% respectively. Conclusion: Corona Virus immunization is reliable and accessible method of controlling the pandemic and as a result reduces it reduces the morbidity and mortality. Pregnant and lactating women need additional knowledge and attitude regarding COVID- 19 vaccination.