Plasmid-mediated resistance gene MCR-1 presence in MDR E. coli represents a paradigm shift in colistin resistance and poses serious threats in colistin use as last resort drug treatment in animal and human health. Following the first MCR- 1 description, researchers around the world published multiple reports about its presence in Enterobacteriaceae. In Morocco, among 560 APEC isolates from septicemic broilers thoroughly scrutinized by the disc diffusion method as an initial screening followed by MIC reference technic, 12 strains were suspected as potential carriers of MCR-1 gene. PCR and Sanger sequencing permitted confirmation of MCR- 1 presence in three strains giving sequence genes with 100 % homology to MCR-1 gene. Results were concordant with multiple reports about chicken suspicions as a potential reservoir for MCR-1 carrying E. coli. Therefore, the use of colistin in animal health must be more controlled and urgent establishment of a national program for antimicrobial resistance surveillance become crucial.