Background: Acinetobacter species has emerged as an important pathogen globally in various infections especially in hospital acquired infections. Objectives: This study was conducted to determine the antibiotic resistance pattern of Acinetobacter species from wound swab samples. Materials and Methods: A Cross sectional study was undertaken in Department of Microbiology, Rajshahi Medical College (RMC), Rajshahi, Bangladesh from period January 2014 to December 2014. A total 13 Acinetobacter were collected from 292 wound infection patients of surgery word and its allied brances in Rajshahi medical college hospital (RMCH). Isolation, Identification and sensitivity of Acinetobacter species were performed by manual method. Results: Out of 292 patients 13(4.4%) patients showed growth of Acinetobacter species. Resistance observed to Meropenem was 38.46%, Piperacillin -Tazobactum 61.53%, Amikacin 53.84%, Ceftazidime 76.92%, Gentamicin 61.53% and Levoflaxacin 67.23%. This data suggest that Acinetobacter isolated from hospital exhibits resistanace to multiple antimicrobial drugs. Conclusion: The study will help to implement better infection control strategies and improve the knowledge of antibiotic resistance patterns of Acinetobacter species in our region.