Dengue is one of the most serious mosquito-borne viral infections affecting tropical and subtropical countries in the world. Since there is no immune prophylactic or specific antiviral therapy available, timely and rapid diagnosis plays a vital role in patient management and implementation of control measures. The present study was planned to diagnose the dengue infection by detecting dengue NS-1 antigen & to study the seroprevalence of dengue NS-1 antigen. Dengue NS-1 antigen testing done by enzyme linked immunosorbent assay (ELISA) was performed during July-2017 to September-2018 and the data were analyzed retrospectively. A total 3397 serum samples received from Guru Gobindsingh Hospital (O.P.D. & Indoor) for the detection of Dengue NS-1 Ag. Out of 3397 samples, total 841 (24.48%) were Positive for Dengue NS1 Ag among this, 548 (65.16%) male and 293 (34.84%) female. And 307 (36.50%) were 0-20 years of age, 448 (53.27%) were 21-40 years of age and 86 (10.23%) were >40 years of age. Present study shows that dengue fever is seen more in 21-40 years of age group and more common in male compare to female. Effective implementation of vector control measures through efforts toward vector breeding source reduction and with the use of personal prophylactic measures against mosquito bites will help in reducing the dengue prevalence in the community.