Urinary tract infections (UTIs) are the most common bacterial infections encountered in clinical practice, which cause morbidity in both hospitalized and out patients. Increasing antimicrobial resistance among bacterial uro-pathogens is a significant health concern. Approximately 1 in 3 women will require antimicrobial treatment for a Urinary Tract Infection (UTI) before age 24, and 40% to 50% of women will have a UTI during their lifetime. UTIs in male patients are considered complicated. Escherichia coli are the most common cause of UTIs. To determine spectrum of microorganisms responsible for UTIs and their antimicrobial sensitive pattern. Clean-Catch midstream urine of the patients was collected. Urine samples received in microbiology laboratory for culture from 881 patients clinically suspected of having urinary tract infection during the study period were processed according to standard protocol. Isolates were identified by conventional phenotypic methods and antibiotic sensitivity determined by standard Kirby Bauer disc diffusion method and follow Clinical and Laboratory Standards Institute (CLSI) guidelines. In this study 191 (21.68 %) patients out of 881 were shown to be urine culture positive out of them 122 (63.87%) females and 69 (36.13%) males. The most isolated bacterium was E. coli with frequency rate of 126 (65.97%). The other bacteria were Klebsiella spp. 35(18.32%), Pseudomonas spp. 18 (9.43%), and Acinetobacter spp. 12 (6.28%). It is concluded that Gram-negative bacilli were responsible for UTI infections in our patients. The most common isolated bacteria from urinary tract infections were E. coli followed by Klebsiella pneumoniae. The most effective antimicrobial agent was Imipenam and the least effective one was cephalexin.