Background: The purpose of this study is to evaluate the epidemiology and analyse the level of antibiotic resistance of Ab strains in the intensive care unit of the ibn Tofail Hospital of the Mohammed VI University Hospital of Marrakech. Results: Microbiologically, 200 samples were analyzed, of which urine was the most frequent sample with a rate of 36% of all samples, followed by blood cultures with a rate of 36%, catheters in 12%, vaginal samples in 7% and protected distal samples in 3%. The analysis of the resistance profile of germs to antibiotics showed that for Beta-lactams: 81% are extended-spectrum beta-lactamases (ESBL) 16% low level penicillinase (LLP) and high level penicillinase (HLP) at 3%.